Comparative characteristics of restreguating properties of spyrocarbon complex with ambic acid and its components

Abstract

Introduction.  Synthesis of new ecologically safe plant growth regulators is one of the urgent agricultural problems. Such drugs are spirocarbon derivatives The complex of spirocarbon with succinic acid belongs to the specified class of synthetic chemicals. Previous studies have suggested the presence of this drug has a biostimulating effect on the process of seedling formation in monocotyledons. The purpose of the study is to prove by means of phytotest "monocotyledonous seedlings" the presence of the above effect in the complex of spirocarbon with succinic acid on the process of seedling formation and to determine the causes of its occurrence.

Materials and Methods.  Phytotest "monocotyledonous seedlings" was made from winter wheat Triticum aestivum L, variety "Asted". Seedlings were formed in Petri dishes at a constant t = 260C according to generally accepted methods. Before germination of seeds its were soaked in the concentration spectrum (10-7-10-2 mol/dm3) of the complex of spirocarbon with succinic acid, their mixture, spirocarbon and succinic acid or in distilled water. At the end of germination in the control and each experimental variant, biometric indicators were determined in representative sample sizes. They included indicators of stem length (hypocotyl), root length and coordination of organ growth. Quantitative data were statistically processed using an Excel resource and a Student's ratio. The probability of differences between the values was estimated by the probability level p = 0.05.

Results and Discussion. Phytotesting of changes in growth rates of seedling organs and coordination of growth of the same organs in experimental and control conditions showed that the least effective drug was a mixture of spirocarbon and succinic acid. Spirocarbon is the next most effective effect on the process of seedling formation. Succinic acid and the complex of spirocarbon with succinic acid had a significant regulatory effect on growth. Both drugs showed this effect at 4-5 concentrations. At the same time, they demonstrated both inhibition and acceleration of seedling growth. Monitoring of changes in the values of coordination of organ growth under the action of all drugs showed differences similar to changes in growth processes. There was no biostimulating effect. A detailed analysis of the obtained results showed that in combination with succinic acid spirocarbon acquires more effective growth-regulating capabilities. The combination of spirocarbon with succinic acid contributes to a 2-fold increase in its restrictive effect. Unequal direction of action of the complex and succinic acid is observed for different organs of the seedling. In coleoptiles, most concentrations of both drugs stimulate growth. At the same time, the same concentrations of the complex inhibit, and succinic acid stimulates root growth. The complex, in contrast to its components, has a biostimulating effect on the growth of seedling organs. The presence of alternation in the spectrum of concentrations that inhibit and accelerate growth is evidence of this. The detected effect has a different direction for the coleoptile and the root of the seedling.

Conclusions. Studies conducted by phytotesting have shown significantly greater efficiency in regulating growth in the complex of spirocarbon with succinic acid than in a mixture of its components. This proves their appearance in the synthesized coordination compound of spirocarbon. The combination of the base substance with succinic acid leads to an increase in the level of these properties not only in spirocarbon, but also in succinic acid. Moreover, the coordination compound of spirocarbon with succinic acid, compared to its components, has new properties - biostimulation of growth. They are demonstrated by monitoring the biometric growth rates of the phytotest "monocotyledonous seedlings" formed from winter wheat Triticum aestivum L. Thus, the synthesis of the complex can be considered as the reason for the acquisition of new properties of spirocarbon and succinic acid. Therefore, the complex compound of spirocarbon and succinic acid is an environmentally friendly drug that has new biological properties compared to its components, and can be recommended for practical use in agriculture.