Influence of CeO2 nanoparticles on seed germination and synthesis of phenols in spruce seedlings

Authors

DOI:

https://doi.org/10.31548/forest2020.03.004

Abstract

Modern technologies make it possible to obtain nanoparticles of biogenic metals for use as an additional source of micronutrient for plants. However, the complexity of mass application of nanosized metal particles and their oxides is due to the significant differences in physicochemical properties of nanocrystalline structures which are dependent on production technology, nanoparticle size, surface charge (z-potential), and stabilization methods. The biocompatibility and nature of nanoparticles has an impact on living organisms.

Regarding the effectiveness and feasibility of using cerium dioxide nanoparticles in crop practice, there is no definitive conclusion. Due to difficulty in the preparation of planting material for seedlings of conifers, the study of the effect of nanocrystalline cerium dioxide on plants is not well researched. The aim of our research was to study the effect of nanocrystalline cerium dioxide solution on the germination of spruce seeds and then to evaluate its effect on the synthesis of phenols as components of the antioxidant system within seedlings.

The research used methods for determining the germination energy and seed similarities.  Other methods used in this research were determining the content of phenolic compounds, flavonoids, and phenolic antioxidants.

The results showed that nanocrystalline cerium dioxide in a concentration of solution from 0.1 to 1.0 mg/mL stimulates the germination of spruce seeds. Under the influence of nanoparticles at a concentration of 0.1 mg/L in the tissues of spruce seedlings increases the content of phenolic compounds. The increase in antioxidant activity of phenols in seedling tissues while decreasing their total amount at a concentration of nanocrystalline cerium dioxide from 0.5 to 1.0 mg/L occurs when increasing the total pool of flavonoids, which are determined by high antioxidant activity.

Nanocrystalline cerium dioxide is a promising material for stimulating germination energy and on the overall germination of spruce seeds.

Keywords: nanoparticles, spruce, seeds, phenols, flavonoids, antioxidants.

References

Asati, A., et al. (2009). Oxidase-Like Activity of Polymer-Coated Cerium Oxide Nanoparticles. Angewandte Chemie, 121(13), 2344-2348. https://doi.org/10.1002/ange.200805279

Cornelis, G., et al. (2011). Solubility and batch retention of CeO2 nanoparticles in soils. Environmental Science & Technology, 45, 2777-2782. https://doi.org/10.1021/es103769k

Corral-Diaz, B., et al. (2014). Cerium oxide nanoparticles alter the antioxidant capacity but do notimpact tuber ionome in Raphanus sativus (L.). Plant Physiol. Biochem., 84, 277-285. https://doi.org/10.1016/j.plaphy.2014.09.018

Garcia, A., et al. (2011). Acute toxicity of cerium oxide, titanium oxide and iron oxide nanoparticles using standardized tests. Desalination, 269, 136-141. https://doi.org/10.1016/j.desal.2010.10.052

Heckert, E. G., Karakoti, A. S., Seal, S., & Self, W. T. (2008). The role of cerium redox state in the SOD mimetic activity of nanoceria. Biomaterials, 29(18), 2705-2709. https://doi.org/10.1016/j.biomaterials.2008.03.014

Ivanov, V.K., Usatenko, A.V., & Shcherbakov, A.B. (2009). Antioxidant activity of nanocrystalline ceria to anthocyanins. Journal of Inorganic Chemistry, 54 (10), 1522-1527 [in Russian]. https://doi.org/10.1134/S0036023609100039

Jabbari, M., & Gharib, F. (2012). Solvent dependence on antioxidant activity of some water-insoluble flavonoids and their cerium (IV) complexes. Journal of Molecular Liquids, 168, 36-41. https://doi.org/10.1016/j.molliq.2012.02.001

Kalenska, S.M., et al. (2012). Scientific substantiation of the use of nanosized biogenic metals in the system of fertilizers for field crops: scientific and practical recommendations. Kyiv. [in Ukrainian].

Likhanov, A.F., Sereda, O.V., Klyachenko, O.L., Melnychuk, M.D. (2018). Influence of oxycoric and oxybenzoic acids on synthesis of plastid pigments and fenolic compounds in the leaves of common grape vine (Vitis vinifera) in vitro. Plant physiology and genetics, 50 (4), 331-343. https://doi.org/10.15407/frg2018.04.331 [in Ukrainian]

Majumdar, S., et al. (2014). Exposure of cerium oxide nanoparticles to kidney bean shows disturbance in the plant defense mechanisms. J. Hazard. Mater, 278, 279-287. https://doi.org/10.1016/j.jhazmat.2014.06.009

Melnychuk, M.D., et al. (2013). Impact of prepearing plant materials on the adapatative potential of scots Pinus sylvestris L. Biological Resources and Nature Management, 5 (5-6), 92-98. [in Ukrainian].

Perez, J.M., Asati, A., Nath, S., & Kaittanis, A. (2008). Synthesis of biocompatible dextran coated nanoceria with pH dependent antioxidant properties. Small, 4, 552-556. https://doi.org/10.1002/smll.200700824

Pinchuk A.P., et al. (2017). The influence of cerium dioxide nanoparticles on germination of seeds and plastic exchange of pine seedlings (Pinus sylvestris L.). Biotechnologia Acta, 10(5), 63-71. https://doi.org/10.15407/biotech10.05.063 [in Ukrainian].

Rico, C. M., et al. (2013). Effect of cerium oxide nanoparticles on rice: a study involving the antioxidant defense system and in vivo fluorescence imaging. Environ. Sci. Technol., 47, 5635-5642. https://doi.org/10.1021/es401032m

Röhder, L.A., Brandt, T., Sigg, L., & Behra, R. (2014). Influence of agglomeration of cerium oxide nanoparticles and speciation of cerium (III) on short term effects to the green algae Chlamydomonas reinhardtii. Aquatic Toxicology. 152. 121-130. https://doi.org/10.1016/j.aquatox.2014.03.027

Schwabe, F., et al. (2013). Influence of two types of organic matter on interaction of CeO2 nanoparticles with plants in hydroponic culture. Chemosphere, 91, 512-520. https://doi.org/10.1016/j.chemosphere.2012.12.025

Shcherbakov, А. B. et al. (2011). Nanomaterials based on the nanocrystalline ceria: properties and use perspectives in biology and medicine. Biotechnology, 4 (1), 9-28. [in Ukrainian].

Sibgatullina_G.V., et al. (2011). Methods for determining the redox status of cultured plant cells. Kazan: Kazan Federal University [in Russian].

Slimestad, R., et al. (1995). Syringetin 3-O-(6″-acetyl)-β-glucopyranoside and other flavonols from needles of norway spruce, Picea abies. Phytochemistry, 40(5). 1537-1542. https://doi.org/10.1016/0031-9422(95)00383-I

SSU 8558:2015 (2015). Seeds of trees and shrubs. Methods for seed testing (germination, viability, benign). Kyiv. [in Ukrainian].

Talankova-Sereda, Т.Ye. (2016). The influence of silicon nanoparticles on Mentha piperita rhizogenesis under in vitro conditions. Taurida Scientific Herald, 96, 112-119. [in Ukrainian].

Trujillo-Reyes, J., et al. (2013). Citric acid modifies surface properties of commercial CeO2 nanoparticles reducing their toxicity and cerium uptake in radish (Raphanus sativus) seedlings. Journal of Hazardous Materials, 263, 677-684. https://doi.org/10.1016/j.jhazmat.2013.10.030

Valgimigli, L., Ingold, K.U., & Lusztyk, J. (1996). Antioxidant Activities of Vitamin E Analogues in Water and a Kamlet-Taft β-Value for Water. Journal of the American Chemical Society, 118(15), 3545-3549. https://doi.org/10.1021/ja954030r

Vincent, A., Inerbaev, T. M., & Babu, S. (2010). Tuning Hydrated Nanoceria Surfaces: Experimental/Theoretical Investigations of Ion Exchange and Implications in Organic and Inorganic Interactions. Langmuir, 26, 7188-7198. https://doi.org/10.1021/la904285g

Wang, Q., Ebbs, S.D., Chenc, Y., & Ma, X. (2013). Transgenerational impact of cerium oxide nanoparticles on tomato plants. Metallomics, 5, 753-759. https://doi.org/10.1039/c3mt00033h

Wang, Q., et al. (2012). The impact of cerium oxide nanoparticles on tomato (Solanum lycopersicum L.) and its implications for food safety. Metallomics, 4, 1105-1112. https://doi.org/10.1039/c2mt20149f

Xu, W., He, H., & Yu, Y., (2009). Deactivation of a Ce/TiO2 catalyst by SO2 in the selective catalytic reduction of NO by NH3. The Journal of Physical Chemistry, 113, 4426-4432. https://doi.org/10.1021/jp8088148

Zaprometov, M. N. (1993). Phenolic compounds. Distribution, metabolism and function in plants. Moskva: Nauka [in Russian]

Zhang Z., et al. (2011). Uptake and distribution of ceria nanoparticles in cucumber plants. Metallomics, 3(8), 816-822. https://doi.org/10.1039/c1mt00049g

Zhao, L., et al. (2012). Effect of surface coating and organic matter on the uptake of CeO2 NPs by corn plants grown in soil: Insight into the uptake mechanism. Journal of Hazardous Materials, 225. 131-138. https://doi.org/10.1016/j.jhazmat.2012.05.008

Downloads

Published

2020-09-30

Issue

Vol. 11 No. 3 (2020)

Section

FORESTRY

License

Relationship between right holders and users shall be governed by the terms of the license Creative Commons  Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).