МІКРОКЛОНАЛЬНЕ РОЗМНОЖЕННЯ POPULUS TREMULA L.
Abstract
MICROPROPAGATION POPULUS TREMULA L.
The peculiarities of microclonal propagation of P. tremula were determined. Way of getting aseptic culture, direct inducing of tissue regeneration of P. tremula, which depending on genotype, type of explants and culture conditions in vitro were researched. Ways of morphogenetic potential realization of micro shoots P. tremula were studied. The optimal components of the culture medium with the addition of 0.25-0.5 mg∙l-1 KIN, TDZ and activated carbon were set. The nutrition mediums provided the full realization of morphogenetic potential, to form explants rooted plants of P. tremula in conditions in vitro and adaptation plants to ex vitro conditions.
Key words: Populus tremula L., micropropagation, nutrient media, explant, morphogenesis, growth regulators, cultivation plantation.
References
Булычёва Н. В. ?зучение влияния сочетаний фитогормонов на каллусообразование и регенеративную способность тополя Populus populus spp. / Н. В. Булычёва, А. М. Камионская, К. Г. Скрябин // Межд. моск. трет. конгр. [«Биотехнология: состояние и перспективы развития»], 14-18 марта, 2005 г. : тез. докл. – Москва, 2005. – Ч. 1 – С. 234.
Прямий органогенез in vitro тирличу жовтого (Gentiana Lutea L.) / [ І. І. Конвалюк, Н. Б. Кравець, Н. М. Дробик та ін. ] // журнал «Біотехнологія». – К. : Інститут біохімії ім. О.В. Палладіна НАН України, 2010. – Т. 3, №5. – С. 66–73.
Кушнір Г. П. Мікроклональне розмноження рослин, теорія і практика / Г. П. Кушнір, В. В. Сарнацька. – К. : Наукова думка, 2005. – 270 с.
Ковалевський С.Б. Культура Populus tremula L.: [монографія] / С. Б. Ковалевський, С. Ю. Білоус, А. Ф. Ліханов. – К.: НУБіП України, 2014. – 189 с.
Ahuja M.R. Aspen. In: Evans DA, Sharp WR and Ammirato PJ (Eds.) Handbook of Plant Cell Culture / M.R. Ahuja / Macmillan Publishing Company. – New York, 1986. – Pp. 626-651.
Aubakirova L. Application of cellular biotechnology for storage of aspen biodiversity (Populus tremula L.) / L. Aubakirova, E. Kalashnikova / International journal of agriculture: Research and review. – 2011. – Vol. 1 (1). – Pp. 16-20.
Driver J. A. In vitro propagation of Paradox walnut root stock / J. A. Driver, A. H. Kuniyuki // HortScience. – 1984. – 19. – P. 507–509.
Murashige T. A revised medium for rapid, growth and bioassays with tobacco tissue cultures / T. Murashige, F. A. Scoog // Physiol. plantarum. – 1962. – Vol. 15. – .№ 3. – P. 473–497.
McCown B. H. Woody plant medium (WP 14) – a mineral nutrient formulation for microculture of woody plant species / B. H. McCown, G. B. Lloyd // Ibid. – 1981. – Vol. 16. – P. 453.
Pijut P. M. Micropropagation of Juglans cinerea L. (Butternut) / P. M. Pijut //Biotechnology in agriculture and forestry. – Berlin: Springer-Verlag, 1997. – V. 39. – P. 345–357.
Tsvetkov I. Thidiazuron-induced regeneration in root segments of white poplar (P. alba L.) / I. Tsvetkov, J. F. Hausman, L. Jouve //. Bulg. J. Agric. Sci. – № 13 – 2007 – P. 623–626 p.
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